Catalog NumberSizePrice (USD)Shopping Cart
EP1000310 ml$19.00Buy Now | Add to Cart
EP1000440 ml.$49.00Buy Now | Add to Cart
Type: Blocking Buffer
Applications: E
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: Not Applicable
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Blocking reagent must to be used for eliminating a non-specific staining in Elisa-spot plates. After coating Elisa-spot plates with capture antibodies blocking buffer must be added to the plate and incubated for 30 min at room temperature. This treatment blocks free binding sites left on the membrane of Elisa-spot plates after coating them with capture antibodies. If un-blocked, detection antibodies, streptavidin-AP and BCIP+NBT reagents will bind producing strong non-specific background staining.